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Consider two fragments of DNA of radius r_1 = 29 nm and r_2 = 36 nm when they ar

ID: 582141 • Letter: C

Question

Consider two fragments of DNA of radius r_1 = 29 nm and r_2 = 36 nm when they are coiled up as they move through a gel. An electrophoresis analysis (see figure below) is carried out to separate these two fragments. Suppose the drag factor C in the equation F_drag = -CrV is C = 5.0 Times 10^-5 kg/(m . s), and assume each fragment has a charge q = -9e. If the electric field has a magnitude of 2.5 N/C, what is the speed of each fragment in the gel? How long will it take the smaller fragment to move a distance 1 cm (a typical distance used in DNA fingerprinting)? After the time in part (b) has elapsed, what is the separation of the two different-size fragments in the electrophoresis tube?

Explanation / Answer

velocity=(EXq/mass)X time

10e-2=1/2(EXq/mass)X time2

calculating we will get the answer.