For the mutation below, (a) characterize the mutation thoroughly, (b) give the a
ID: 58448 • Letter: F
Question
For the mutation below, (a) characterize the mutation thoroughly, (b) give the amino acid sequence resulting from the mutated strand and, (c) state what effect the mutation may have on the function of the gene product
Mutation 1: 3’ g t c A C A G G T T T A c a c g t t a a a c a t c G C G A A G T T T 5’
(a) characterize the mutation thoroughly (6 points)
(b) give the amino acid sequence resulting from the mutated strand (6 points)
(c) state what effect the mutation may have on the function of the original gene product (8 points)
Explanation / Answer
The following table is normal sequence
After removing G at 7th position, the following is the sequence of amino acids.
Due to deletion of G at 7th position causing frame shift mutation that lead to nonsense mutation, which results incomplete protein yielding
DNA 3' GTC ACA GGT TTA CAC GTT AAA CAT CGC GGA GTT T5' Mrna CAG UGU CCA AAU GUG CAA UUU GUA GCG CCU CAA A amino acids Glu Try Pro Asn Val Pro Phe Val Ala Leu Glu