For the mutation below, (a) characterize the mutation thoroughly, (b) give the a
ID: 58471 • Letter: F
Question
For the mutation below, (a) characterize the mutation thoroughly, (b) give the amino acid sequence resulting from the mutated strand and, (c) state what effect the mutation may have on the function of the gene product
Mutation 1: 3’ g t c A C A G G T T T A c a c g t t a a a c a t c G C G A A G T T T 5’
(a) characterize the mutation thoroughly (6 points)
(b) give the amino acid sequence resulting from the mutated strand (6 points)
(c) state what effect the mutation may have on the function of the original gene product (8 points)
Explanation / Answer
a. Nonsense mutation
b. Gln-Cys-Pro-Asn-Val-Gln-Phe-Val-Ala-Leu-Gln, bold indicates the amino acids coded by mutated nucleotides (small letters). You did mention that whether we have to start from starting codon or not. There is one nucleotide is left is not coming into amino acid coding since, 34 nucleotides (11x3=33nucleotides) for 11 amino acids. You suppose to provide full details.
c. Out of 11 amino acids, 6 were mutated, and 5 were normal. This is abnormal and may be the protein become non-functional and structurally defaulted.