Please answer and explain the following questions from the image below: You are
ID: 60220 • Letter: P
Question
Please answer and explain the following questions from the image below:
You are interested in understanding the gene regulation of Lkp1, a protein that is normally produced in liver and kidney cells in mice. Interestingly, you find that the LKP1 gene is not expressed in heart cells. You isolate the DNA upstream of the LKP1 gene, place it upstream of the gene for green fluorescent protein (GFP), and insert this entire piece of recombinant DNA into mice. You find GFP expressed in liver and kidney cells but not in heart cells, an expression pattern similar to the normal expression of the LKP1 gene. Further experiments demonstrate that there are three regions in the promoter, labeled A, B, and C in the Figure below, that contribute to this expression pattern. Assume that a single and unique transcription factor binds each site such that protein X binds site A, protein Y binds site B, and protein Z binds site C. You want to determine which region is responsible for tissue-specific expression, and create mutations in the promoter to determine the function of each of these regions. In the Figure below, if the site is missing, it is mutated such that it cannot bind its corresponding transcription factor. Which of the following proteins is likely to act as a gene repressor? In what tissue is factor Z normally present and bound to the DNA?Explanation / Answer
14. c. factor Z is the gene repressor.
15. c. Heart
We can observe that mutation in promoter region C is associated with its expression in heart.