Polyadenylation of mRNA requires all of the following EXCEPT: A) Pol II extends

Question

Polyadenylation of mRNA requires all of the following EXCEPT: A) Pol II extends the transcript beyond the site where the poly(A) tail is added. B) The site of poly(A) addition is marked by specific sequence elements in the transcript. C)The transcript is cleaved by an endonuclease that associates with the C-terminal domain of Pol II. D) Polyadenylate polymerase uses a poly(U) RNA template to synthesize the poly(A) tail. Polyadenylation of mRNA requires all of the following EXCEPT: A) Pol II extends the transcript beyond the site where the poly(A) tail is added. B) The site of poly(A) addition is marked by specific sequence elements in the transcript. C)The transcript is cleaved by an endonuclease that associates with the C-terminal domain of Pol II. D) Polyadenylate polymerase uses a poly(U) RNA template to synthesize the poly(A) tail.

Explanation / Answer

Polyadenylation of mRNA requires all of the following EXCEPT: Polyadenylate polymerase uses a poly(U) RNA template to synthesize the poly(A) tail. The site for the polyadenylation contains a signal sequence AAUAAA on the RNA. Hence, option D is correct.

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