Please answer and explain the following question (#22) below. It comes with some
ID: 60352 • Letter: P
Question
Please answer and explain the following question (#22) below. It comes with some introductory information that may be useful, which I have included. Thank you!
The Drosophila Eve gene has a complex promoter containing multiple binding sites for four transcription regulators: Bicoid, Hunchback, Giant, and Krüppel. Bicoid and Hunchback are activators of Eve transcription, whereas Giant and Krüppel repress Eve transcription. Figure A shows the patterns of expression of these regulators. Figure B shows the module for stripe 2, 3 and 7 upstream of the eve gene. stripe 3 stripe 2 stripe 7 module module Eve stripe 2 TATA eve gene Krüppel Bicoid (bcd) stripe 2 module: 480 nucdeotide pairs anterior pole pole Krüppel and its binding site Bicoid and its binding site Giant and its binding site Hunchback and its binding site stripe numbers 2 3 -galactosidase expression pattern ripe numbers stripe numbers (a) NORMAL (C) MUTANT (b) MUTANT (d) MUTANT anterio posterior The Eve promoter contains modules that control expression in various stripes. You construct a reporter gene that contains the DNA 5 kb upstream of the Eve gene, so that this reporter contains the stripe 3 module, the stripe 2 module, the stripe 7 module, and the TATA box, all fused to the LacZ reporter gene (which encodes the B-galactosidase enzyme, as shown in Figure B. This construct results in expression of the -galactosidase enzyme in three stripes, which correspond to the 1 normal positions of stripes 3, 2, and 7 You make a mutant version in which the Krüppel-binding sites in stripe 2 module have been deleted. Refer to Figure B for the positions of the binding sites, 22. Match the appropriate mut ate mutant condition with the most likely pattern of Eve expression shown in Figure C. Choose a letter (a - d) from the 4 picture choices in Figure C. Explain your choices.Explanation / Answer
C. The answer would be most likely (d). Since Kruppel represses eve transcription in normal condition, a mutant with no Kruppel would promote the transcription of eve genes and thus the band width would increase.