In his analysis of rll in phage, famous scientist Benzer assayed complementation
ID: 61354 • Letter: I
Question
In his analysis of rll in phage, famous scientist Benzer assayed complementation by coinfecting E. coli K12 with pairs of phage mutants. Suppose you try your hand at this with several mutants and get the following results (+ = complementation, ie. production of functional phage at high frequency; each mutant is denoted by a number): Mutant 42 and mutant 62 Mutant 42 and mutant 83 Mutant 62 and mutant 63 - Mutant 62 and mutant 74 + Mutant 42 and mutant 74 Mutant 42 and mutant 75 Mutant 62 and mutant 75 What can you conclude? 1 point) O O O O O Mutations 42 and 75 are in the same gene. Mutations 62 and 74 are in the same gene. Mutations 62, 63, and 74 are in the same gene Mutations 42 and 74 are in the same gene. Mutation 63 is in a gene with neither 62 nor 42.Explanation / Answer
The answer is Mutations 42 and 74 are in the same gene.
When the mutations present on different genes of phage are co-infected, then the two genes will complement to form the plaques. Two genes present on same gene will not complement.