The following sequence represents a eukaryotic gene found between a promoter and
ID: 64384 • Letter: T
Question
The following sequence represents a eukaryotic gene found between a promoter and terminator region. The top strand is the coding strand and the bottom strand is the template strand. Using the attached genetic code, complete the following. Note, the bold region indicates intron regions.
a. What is the sequence of the primary (initial) transcript - describe or draw using the supplied sequence below b. What is the sequence of the processed transcript - be sure to include mRNA modifications found in eukaryotes
c. Underline the 5’ and 3’ untranslated regions on the provided DNA sequence (coding strand only)
d. What is the amino acid sequence of the polypeptide encoded by this gene - indicate the N terminus and C terminus
e. A mutation that results in a substitution of an A for the C at the indicated position (*) doesn’t change the final amino acid sequence. Name the phenomenon that allows for this result.
f. The silent point mutation described above involves a substitution of a C to an A. What type of substitution is this (use the appropriate term) *
5’-GAACCGATCTGATGGTCCAGCAATCAAGAGTTACCGACTCTG TAGGATGCCCTAGAATAC-3’
3’-CTTGGCTAGACTACCAGGTCGTTAG TTCTCAATGGCTGAGACATCCTACGGGATC TTATG-5’
The genetic code Second letter 3 UGA Stop UAG Stoip CUU CCU eu CAA CCG ACU AAU AUU AUA Asn AGA Arg AGG AUG Met ACG GCU GCC GGU GUU G GUC GUG GCGExplanation / Answer
1. Primary transcript is a single stranded RNA sequence transcribed from DNA.
5’-GAACCGATCTGATGGTCCAGCAATCAAGAGTTACCGACTCTG TAGGATGCCCTAGAATAC-3’ (coding strand)
5’-CUUGGCUAGACUACCAGGUCGUUAGUUCUCAAUGGCUGAGAC AUCCUACGGGAUCUUAUG-3’ (Coding strand) ss RNA strand