The following sequence represents the DNA template strand of a gene: 3\' - TAC C
ID: 7455 • Letter: T
Question
The following sequence represents the DNA template strand of a gene:
3' - TAC CGT GTC TCC CTT GAT ATT GAA CTA - 5'
AUG GCA CAG AGG GAA CUA UAA CUU GAU
Give the amino acid sequence of the protein encoded by this sequence after the following mutations have occurred:
• a transition at nucleotide #10
• a transition at nucleotide #12
• a transition at nucleotide #7
• an insertion of a “G” immediately following nucleotide #10
• a three base pair insert (of your choice) starting with base 5
• What kind of mutated (four bases instead of the typical three) “pop” Amino Acid is created by inserting a G between base 14 and 15 of the original RNA strand
Explanation / Answer
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The following sequence represents the DNA template strand of a gene:
3' - TAC CGT GTC TCC CTT GAT ATT GAA CTA - 5'
AUG GCA CAG AGG GAA CUA UAA CUU GAU
by transcription
DNA--->mRNA
3' —TAC CGT GTC TCC CTT GAT ATT GAA CTA—5'
PRODUCES
AUG GCA CAG AGG GAA CUA UAA CUU GAU
Met Ala- Gln Arg- Glu -Leu
UAA STOP CODON
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• a transition at nucleotide #10
3' —TAC CGT GTC TCC CTT GAT ATT GAA CTA—5'
3' —TAC CGT GTC CCC CTT GAT ATT GAA CTA—5'
AUG GCA CAG GGG GAA CUA UAA CUU GAU
Met Ala- Gln GLY- Glu -Leu
code for glycine.
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• a transition at nucleotide #12
3' —TAC CGT GTC TCC CTT GAT ATT GAA CTA—5'
AUG GCA CAG GGG GAA CUA UAA CUU GAU
no change in the sequence.
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• a transition at nucleotide #7
So codon CAG would be UAG.
UAG. stop codon.
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an insertion of a “G” immediately following nucleotide #10
ANSWER :Thr
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MAY BE FRAME SHIFT