CHAPTER 5 QUESTIONS 1. Look at the results of your transformation. Do your actua
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Question
CHAPTER 5 QUESTIONS
1. Look at the results of your transformation. Do your actual results match your predicted results? If not, what differences do you see, and what are some explanations for these differences?
2. How many red colonies were present on your LB/amp/ara plate?
3. Why did the red colonies only appear on the LB/amp/ara plate and not the LB/amp plate?
4. Recombinant plasmids are engineered so that they can replicate in the cell independently of the chromosome replication. Why is it important to have multiple copies of a recombinant plasmid within a cell?
5. How is the information encoded in the rfp gene expressed as a trait? Be sure to use what you have previously learned about gene expression and the relationship between DNA, RNA, protein, and traits.
6. Why is it possible for bacteria to make a human protein, such as insulin, or a sea anemone protein, such as the red fluorescent dye?
7. The only bacteria that could produce the red fluorescent protein in Laboratory 5 were bacteria that were transformed with the pARA-R plasmid. Why?
Explanation / Answer
3. The red colonies would have only appeared on the LB/amp/ara plate because araganose is required to express the red protein.
4. Having multiple copies of plasmid allows the bacteria to better express the gene of interest.
5. The central dogma of molecular biology is DNA>RNA>protein. The expression happens when the DNA is transcribed to RNA, and then translated to protein.
6. The central dogma of molecular biology is same for all organisms, and the codons are also almost similar in all the organisms. These factors made it possible to express any protein from any organism using different bacteria.