Students were asked the following question: What is the difference between rever
ID: 64935 • Letter: S
Question
Students were asked the following question: What is the difference between reversible inhibition& negative allosteric regulation? Student #1 wrote (the sentence are numbered for easy reference): (1) Negative allosteric regulation does not follow Michaelis-Menten kinetics. (2) If Ko.5 stays the same, Vmax will increase and the velocity curve will be hyperbolic. (3) Reversible inhibition affects catalysis, by reducing the velocity. (4) Therefore, Vmax may decrease or stay the same, and Km will always increase. What (if anything) would you CHANGE, ADD, or DELETE from each statemetn to make it correct?Explanation / Answer
Negative allosteric regulation requires a ligand which bind to site other than active site ,which there by decreases the efficiency of enzyme by decreasing the affinity for the substrate . They do not follow michelis menton constant.
Reversible inhibhition ; The inhibhitors bind noncovalently ,bind to the active site in place of the substrate may involve adding or removing phosphate groups,It affects the catalysis by decreasing the velocity ,therefore V max may reduce .