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Could someone please explain how to get the answer and possible work it for me?

ID: 66008 • Letter: C

Question

Could someone please explain how to get the answer and possible work it for me? Thanks in Advance!

In yeast, ethanol is produced from glucose under anaerobic conditions. What is the maximum amount of ethanol (in millimoles) that could theoretically be produced under the following conditions? A cell-free yeast extract is placed in a solution that contains 325 mmol glucose, 0.40 mmol ADP, 0.40 mmol P¡, 0.80 mmol ATP, 0.20 mmol NAD^+, and 0.20 mmol NADH. It is kept under anaerobic conditions. Under the same conditions, what is the theoretical minimum amount of glucose (in millimoles) required in the solution to form the maximum amount of ethanol?

Explanation / Answer

C6H1206 2 CH3CH2OH + 2 CO2+ 2ATP

There are two molecules of ethanol produced from one molecule of glucose.

It is visible that NAD is limiting substrate in solution.

Therefore after the exhaustion of NAD the reaction will stop.

Therefore the maximum production of ethanol via glucose will be 2X 0.20mmol

= 0.4mmol.

b. Theoretically mininum 0.20 mmol of glucose was enough for max production of ethanol in this condition.