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Please explain in detail the below. Third time posting as previous answers only

ID: 67439 • Letter: P

Question

Please explain in detail the below. Third time posting as previous answers only provide answers and not explanation. Seeking explanation not only answer.

The data below comes from a young couple who both have siblings with a genetic disorder of amino acid metabolism. The amino acid pathway that is disordered has 4 enzymes coded for by 4 independently assorting genes. You have the RFLP data collected from the couple (C1 and C2) their parents and their affected sibs (C1’s parents are P1 and P2 and the affected sib is S1) (C2’s parents are P3 and P4 and her affect sib is S2). Their parents are unaffected by the disorder. Each gene has an RFLP that is linked to the gene.

1) Based on this data is there a significant chance a child born to C1 and C2 will have the disorder? Explain why.

2) Are C1 or C2 carriers for the disorder? Explain why.

3) What is the probability a child born to C1 and C2 could be a double?

RFLP FOR GENE 1

size

P1

P2

S1

C1

C2

S2

P3

P4

14kb

X

X

X

X

X

10kb

X

X

X

X

8kb

X

X

X

4kb

X

X

X

RFLP FOR GENE 2

size

P1

P2

S1

C1

C2

S2

P3

P4

16kb

X

X

X

X

X

10kb

X

X

X

X

6kb

X

X

X

2kb

X

X

X

RFLP FOR GENE 3

size

P1

P2

S1

C1

C2

S2

P3

P4

20kb

X

X

X

X

X

15kb

X

X

10kb

X

X

X

X

X

X

45Kb

X

X

RFLP FOR GENE 4

size

P1

P2

S1

C1

C2

S2

P3

P4

12kb

X

X

X

X

10kb

X

X

X

X

X

X

X

8kb

X

X

6kb

X

size

P1

P2

S1

C1

C2

S2

P3

P4

14kb

X

X

X

X

X

10kb

X

X

X

X

8kb

X

X

X

4kb

X

X

X

Explanation / Answer

Since the concerned disordered is autosomal recessive then definitely, both the parents of the affected individual would be the carrier and the relevant data supports the same. The RFLP relevant data clearly suggests that -

If we talk about gene1, C1 inherited 14 kb & 10 kb band from each parent (P1 and P2) and C2 inherit 14 & 4 kb band from P3 and P4 respectively.

Similarly, regarding Gene 2 C1 inherited 10 kb & 6 kb band from each parent and C2 inherit 16 & 2 kb band.

regarding gene 3, C1 inherited 20 kb & 10 kb band from each parent and C2 inherit 20 & 10 kb band, which is quite significant.

regarding gene 4, C1 inherited 12 kb & 10 kb band from each parent and C2 inherit 12 & 10 kb band which is quite significant.