I need help with these genetic procedure I don\'t know how I do it Bioinformatic
ID: 69161 • Letter: I
Question
I need help with these genetic procedure I don't know how I do it Bioinformatics: Comparing Sequences Evolutionary Relationships, Phylogenetic Trees Procedure 1. Log on to Biology WorkBench (http://workbench.sdsc.edu/) the server at the University of California at San Diego, click on "Enter Biology WorkBench 3.2", enter a logon name and password. 2. When the new screen appears click on "Session Tools" 3. When the new screen appears highlight "Start New Session" and then click on "Run" 4. On the next screen enter "influenza" as your session description and then click on Start New Session 5. On the next screen, select "Nucleic Acid Tools",highlight "Add New Sequence", and click on Run 6. Paste in the first influenza sequence in the box provided, label "HINI" followed by the date of the sequence C"HINI 1918", for example), and "Save" 7. On the next screen highlight "Add New Sequence" and click on "Run". Continue adding sequences until all of your influenza sequences have been added Rooted Phylogenetic Tree S. When all sequences have been added, check all the boxes next to each file (except for the H3N2 sequence), scroll down the menu, select-CLUSTAL W", and click on "Run" 9. On the next screen, change the default from an "Unrooted Tree" to a "Rooted Tree" and elick orn submit. The results will show the conserved sequences in blue and the unconserved sequences in black. Gaps in the sequence are shown as dashes. A rooted phylogenetic tree will also be shown. Notice that the recent HINI influenza is more closely related to the 1918 and 1935 strains than it is to the more recent 2007 and 2001 strains. HIN1 200 -H1N1 1991 -HINI 1918 _ HINI 1935 H1N1 2007 H1N1 2001 _HINI 1982 HIN1 1978 HIN1 1957 LlLH1N1 1951 _HINI 1940 HINT 1945 HIN1 1945Explanation / Answer
You need to add each sequence with the help of add new sequence there after click on Run. Keep adding sequence until all of your influenza sequences have been added.