Topic: nucleotide Can someone please help me answer the questions above and expl

Question

Topic: nucleotide

Can someone please help me answer the questions above and explain why did you pick that answer?

Thanks

Association of the beta clamp with Pol I would effect: Leading strand synthesis Polymerase would replace longer stretches of DNA Efficiency of Okazaki fragment production Polymerase accuracy, so 3'rightarrow 5' exonuclease would digest more DNA Decrease in polymerase activity What are the main reasons for the high fidelity of DNA Polymerase III? 3'-5' exonuclease, beta-clamp, and helicase 5'-3' exonuclease, topoisomerase, and base-pairing balanced NTP's, base-pairing (ionic interactions), and 3'-5' exonuclease SSB, Pol I, and ligase balanced dNTP's, base-pairing (hydrogen bonding/shape), and 3'-5' exonuclease

Explanation / Answer

Answer1: With best of knowledge, beta clamp is generally assocaited with DNA PolII and its main funstion is to form pre intition complex at DNA strand to start the DNA synthesis at leading strand. Therefore option A is correct.

Answer 2: Option A is correct. It not helps in 3-5 exocnuclease activity but also helps in beta clamp formation for replosome assembly.

DNA Pol III enzymes, each comprises , and subunits. The subunit (encoded by the dnaE gene) has the polymerase activity. the subunit (dnaQ) has 3'5' exonuclease activity. The subunit (holE) stimulates the subunit's proofreading.

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