Topic: nucleotide Can someone please help me answer the questions above and expl

Question

Topic: nucleotide

Can someone please help me answer the questions above and explain why did you pick that answer?

Thanks

Why is a heat stable polymerase critical to the success of DNA amplification in the PCR reaction? none of the below required to denature the DNA and anneal the primer contains 5' to 3' exonuclease to excise the primers it is extremely processive and can duplicate long stretches of DNA can undergo the multiple rounds of cooling cycles to denature DNA an anneal primers The fundamental reason that RFLP analysis can uniquely identify an individual's DNA fingerprint is exonucleases activity single nucleotide polymorphisms between people point mutations produce different chromosomal lengths differentia] specificity of restriction endonucleases differential gene expression patterns

Explanation / Answer

1. B. In the process of PCR, the temperature is raised so that the DNA is denatured into single strands. These single strands then hybridize with the primers in the solution. So, the enzyme has to be able to bear the high temperatures and also work efficiently.

2. B. The presence of SNPs in a individual is unique and hence helps in DNA tests.

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