Please Help The most accurate values of absorbance are obtained in the range of
ID: 709795 • Letter: P
Question
Please Help
The most accurate values of absorbance are obtained in the range of A = 0.3-2. Which of the following reasons account for lowered accuracy at higher or lower absorbancy values? (Check all that apply). Beer's law is not followed at very high absorbance values. Too little difference between sample and reference cell if absorbance is very high. Too little light reaches the detector if absorbance is very high. Too little light reaches the detector if absorbance is very low. Too little difference between sample and reference cell if absorbance is very low. Beer's law is not followed at very low absorbance values.Explanation / Answer
If absorbance is too high, too little light reaches the detector for accurate measurement. If absorbance is too low, there is too little difference between sample and reference for accurate measurement.