Part A You have discovered a novel protein that has a pI-5.4. To study the funct

Question

Part A You have discovered a novel protein that has a pI-5.4. To study the functional properties of this new protein, your research group has made a mutant that contains two amino acid changes-namely, a surface Phe residue in the normal protein has been replaced by His (side chain pK,-6.1), and a surface Gin has been replaced by Glu (side chain pK-4.2) ls the pl of the mutant protein predicted to be greater than less than, or the same as the pl of the normal pr O The pl of the mutant protein predicted to be greater than the pl of the nomal protein. O The pl of the mutant protein predcted to be less than the pl of the normal protein O The pl of the mutant protein predicted to be the same as the pl of the normal protein Correct Part B Support your answer with the calculation of the charge on the mutant at pH 5.4 Express your answer using two decimal places. Submit Previeus Answers Reqvest Answer

Explanation / Answer

a)

pI = 1/2 ( 6.1 + 4.2)

pI = 5.15

so it is less than normal protein

b) at pH = 5.4

since the pH is greater than the isoelectric point so the protein has a net negative charge.

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