I\'ve already asked this question the answer was incomplet/confusing, so only an
ID: 72435 • Letter: I
Question
I've already asked this question the answer was incomplet/confusing, so only answer if you can actualyl draw out part D and explain. (I got A.) P B.) P&A C.) P&A D.) E&P by the way.. :)
*** ONLY NEED PART D don't answer unless you can do all 3 steps of part 3
Using the diagrams below, indicate the position of tRNAs
(A) during tRNA selection before GTP hydrolysis by EF-Tu,
(B) after GTP hydrolysis by EF-Tu and proofreading,
(C) after peptide bond formation but before translocation, and
(D) after translocation by EF-G. ****Indicate the tRNAs as straight lines and the nascent peptide by a squiggly line. ****Which one of these represents the hybrid state? ****Finally, indicate which steps involve GTP hydrolysis.
Explanation / Answer
D. EF-G is a GTAase responsible for catalyzing the coordinated movement of tRNA and mRNA through the ribosome. EF-G causes the tRNA that is previously situated in either A or P position to shift from P to E or A to P.
So, after translocation by EF-G, the tRNA bound at the A site moves to P site, and tRNA that is bound to P site moves to E site.
The tRNA shifted from P to E site is generally considered as P/E hybrid state.
Translocation step where tRNA moves from P site to E site requires GTP hydrolysis.
Hope you will understand this time.