Carbonic Anhydrase (MW= 30,000) catalyzes the reversiblehydration of CO 2 . If 1

Question

Carbonic Anhydrase (MW= 30,000) catalyzes the reversiblehydration of CO2. If 10.0 %u03BCg pure carbonicanhydrase catalyzes the hydration of 0.30 g of CO2 in 1min at 37 C at Vmax, what is the turnover number(kcat) of carbonic anydrase (in units ofmin-1)? Carbonic Anhydrase (MW= 30,000) catalyzes the reversiblehydration of CO2. If 10.0 %u03BCg pure carbonicanhydrase catalyzes the hydration of 0.30 g of CO2 in 1min at 37 C at Vmax, what is the turnover number(kcat) of carbonic anydrase (in units ofmin-1)?

Explanation / Answer

2.3 g of CO2 = 2.3/(12+32) = 2.3/44 mol = 3.148*10^22 molecules

Per second = 3.148*10^22/300 = 1.05*10^20 sec-1 per 50 micrograms.

Weight of enzyme ~ 40000 daltons or 40000 g/mol

So, moles in 50 micrograms ~ 50*10^(-6)/40000 = 1.25*10^-9 mol

So, kcat or turnover number

41818182 or 4.2*10^7

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