I need help with these questions. I\'ve answered the rest. A sample of Fe3+ trav
ID: 799152 • Letter: I
Question
I need help with these questions. I've answered the rest.
A sample of Fe3+ traveled a distance of 75 nm. The mobile phase consisted of a mixture of 2 butanone and HCl The solvent front traveled a distance of 83 mm. Calculate the Rf of the iron(III). In a second chromatogram the solvent front traveled 90mm. If iron(III) were present at what distance would you expect to see a spot? If a solute has a large affinity for the mobile phase and travels the same distance as the solvent front what will be the value of its Rf? How will you select an appropriate spot test reagent for each cation? What are the hazards associated with the developing solution that contains hydrochloric acid and acetone.Explanation / Answer
1. Rf = 75/83 = .54
2. distance of Fe3+ = (.54)(90nm) = .48 nm
3. The Rf value would be 1, however this is not an accurate experiment. There must be a difference in the distance traveled by the mobile phase and the distance traveled by the solvent front to be a working PCR plate.
4. http://genchem.rutgers.edu/spot.html
Here is a list explaining how to select a spot test reagent.
5. Hydrochloric acid is poisonous and corrosive. It can cause severe burns if it comes in contact with the skin. It can be fatal if swallowed or inhaled. Inhalation may cause lung damage.
Acetone can irritate skin, eyes, internal organs. Repeated or prolonged exposure can cause organ damage.