An enzyme catalyzes a reaction at a rate of 18 ?mol/min when the substrate conce
ID: 819803 • Letter: A
Question
An enzyme catalyzes a reaction at a rate of 18 ?mol/min when the substrate concentration is 10 mM. The Km of the enzyme for this substrate is 1 An enzyme catalyzes a reaction at a rate of 18 ?mol/min when the substrate concentration is 10 mM. The Km of the enzyme for this substrate is 1 An enzyme catalyzes a reaction at a rate of 18 ?mol/min when the substrate concentration is 10 mM. The Km of the enzyme for this substrate is 1 times 10-5 M. Determine what the reaction velocity will be when the substrate concentration is 1 times 10-5 M.Explanation / Answer
The velocity of 18 ?mol/min is essentially Vmax because it is measured at [S] >> Km. (a) When [S] =
10-5 M = Km, V = 1/2 Vmax, = 9 ?mol/min.