Show all work to receive credit. (A) calculate delta G prime for the last reacti
ID: 821486 • Letter: S
Question
Show all work to receive credit.
(A) calculate delta G prime for the last reaction of glycolysis.
(B) calculate the equilibrium ratio of [products]/[reactants] at 298K for the last reaction of glycolysis using your answer to (A).
(C) Delta G for the last reaction of glycolysis is listed as -16.7 Kj/mol. Calculate the actual ratio of [products]/[reactants]. (Use your answer to A). Compare this answer to your answer to (b).
(D) using your answer to (C) if the ratio of pyruvate to phosphoenolpyruvate is 30, what would be the ratio of [ATP]/[ADP].
(E) calculate delta G prime for the reverse of the last glycolysis reaction in gluconeogenesis, assuming that deltaG prime for the hydrolysis of GTP=deltaG prime for hydrolysis of ATP. Use your answer to (A).
The net reaction is: Pyruvate+ATP+GTP+H2O --><-- phosphoenolpyruvate +ADP+GDP+Pi
Explanation / Answer
#
Reaction
Enzyme
ATP Yield
delta Go' (kJ/mol) (kcal/mol)*
delta G (kJ/mol) (kcal/mol)*
1
Glucose --> G-6-P
2
G-6-P --> F-6-P
3
F-6-P --> F-1,6-bisP
4
F-1,6-bisP --> Galde-3-P + DHAP
5
DHAP --> Galde-3-P
6
2(Galde-3-P) --> 2(G-1,3-bisP) & 2(G-1,3-bisP) --> 2(G-3-P)
Glyceraldehyde-3-P Dehydrogenase & Phosphate Glycerate Kinase
8
2(G-3-P) --> 2(G-2-P)
9
2(G-2-P) --> 2PEP
10
2PEP --> 2Pyr
Net
+2
-43.3
-74.0
Delta G values are estimated from approx. intracellular conc. of glycolytic intermediates in heart muscle.
delta G = -16.9 +30 = 13.1 kJ/mol
Summary of Glycolytic Reactions#
Reaction
Enzyme
ATP Yield
delta Go' (kJ/mol) (kcal/mol)*
delta G (kJ/mol) (kcal/mol)*
1
Glucose --> G-6-P
-1-20.9 (-4.0)*
-27.2 (-8.0)*
2
G-6-P --> F-6-P
0+2.2 (+0.4)*
-1.4 (-0.6)*
3
F-6-P --> F-1,6-bisP
-1-17.2 (-3.4)*
-25.9 (-5.3)*
4
F-1,6-bisP --> Galde-3-P + DHAP
0+22.8 (+5.7)*
-5.9 (-0.3)*
5
DHAP --> Galde-3-P
0+7.9 (+1.8)*
+4.4 (+0.6)*
6
2(Galde-3-P) --> 2(G-1,3-bisP) & 2(G-1,3-bisP) --> 2(G-3-P)
Glyceraldehyde-3-P Dehydrogenase & Phosphate Glycerate Kinase
0-16.7 (+1.5)*
-1.1 (-0.4)*
8
2(G-3-P) --> 2(G-2-P)
0+4.7 (+1.1)*
-0.6 (+0.2)*
9
2(G-2-P) --> 2PEP
0-3.2 (+0.4)*
-2.4 (-0.8)*
10
2PEP --> 2Pyr
+2-23.0 (-7.5)*
-13.9 (-4.0)*
Net
+2
-43.3
-74.0