For the following coding DNA sequence: CAACATGGTATGTGGCAACATGGACCCAGAATGTGACTTCA
ID: 82776 • Letter: F
Question
For the following coding DNA sequence:
CAACATGGTATGTGGCAACATGGACCCAGAATGTGACTTCATCACCCAGCTGAGAGAGGA
TGAGAGTGCCTGTCTACAAGCAGCAGAGGAGATGCCCAACACCACCCTGGGCTGCCCTGC
GACCTGGGATGGGCTGCTGTGCTGGCCAACGGCAGGCTCTGGCGAGTGGGTCACCCTCC
CCTGCCCGGATTTCTTCTCTCACTTCAGCTCAGAGTCAGGGGCTGTGAAACGGGATTGTAC
TATCACTGGCTGGTCTGAGCCCTTTCCACCTTACCCTGTGGCCTGCCCTGTGCCTCTGGA
GCTGCTGGCGTGAGGAGG
Determine the mRNA and amino acid sequences that this DNA codes for
In mutant X, base 35 is C instead of G. What would be the resulting amino acid sequence, and would this protein likely be functional?
In mutant Y the first G of the sequence is deleted. What would be the resulting amino acid sequence, and would this protein likely be functional?
Explanation / Answer
mRNA sequence of gene:
CAACAUGGUAUGUGGCAACAUGGACCCAGAAUGUGACUUCAUCACCCAGCUGAGAGAGGAUGAGAGUGCCUGUCUACAAGCAGCAGAGGAGAUGCCCAACACCACCCUGGGCUGCCCUGCGACCUGGGAUGGGCUGCUGUGCUGGCCAACGGCAGGCUCUGGCGAGUGGGUCACCCUCCCCUGCCCGGAUUUCUUCUCUCACUUCAGCUCAGAGUCAGGGGCUGUGAAACGGGAUUGUACUAUCACUGGCUGGUCUGAGCCCUUUCCACCUUACCCUGUGGCCUGCCCUGUGCCUCUGGAGCUGCUGGCGUGAGGAGG
In this mRNA, if firs three bases considered as triplet than translation will start at 10th base due to presence of ATG sequence that code for Methinine amini acid. This sequence will terminate after synthesizing 8-amino acids that is due to presence of termination codon in DNA sequence. That termination is present at 35th base of given sequence. Amino acid sequence is as follow:
QHGMWQHGPRM& (termination due to 35th base pair)
LHHPAERG&
ECLSTSSRGDAQHHPGLPCDLGWAAVLANGRLWRVGHPPLPGFLLSLQLRVRGCETGLYYHWLV&
ALSTLPCGLPCASGAAGVRR
Note:In this sequence & represent termination of protein.
In this question Bold M represent where the translation will begin. Bold and increases font size represent change amino acid.
In first situation where 35th base is replace by the C.
mRNA sequence :
CAACAUGGUAUGUGGCAACAUGGACCCAGAAUGUCACUUCAUCACCCAGCUGAGAGAGGAUGAGAGUGCCUGUCUACAAGCAGCAGAGGAGAUGCCCAACACCACCCUGGGCUGCCCUGCGACCUGGGAUGGGCUGCUGUGCUGGCCAACGGCAGGCUCUGGCGAGUGGGUCACCCUCCCCUGCCCGGAUUUCUUCUCUCACUUCAGCUCAGAGUCAGGGGCUGUGAAACGGGAUUGUACUAUCACUGGCUGGUCUGAGCCCUUUCCACCUUACCCUGUGGCCUGCCCUGUGCCUCUGGAGCUGCUGGCGUGAGGAGG
If 35th base replace by C than it code for serine amino acid instead of terminator.
QHGMWQHGPRMSLHHPAERG&
ECLSTSSRGDAQHHPGLPCDLGWAAVLANGRLWRVGHPPLPGFLLSLQLRVRGCETGLYYHWLV&ALSTLPCGLPCASGAAGVRR
Now this protein synthesizes 17 amino acids where first three DNA/RNA base considered for first codon.
In second case first G of the sequence is deleted.
First G is present at 7th position:
DNA sequence
CAACATGTATGTGGCAACATGGACCCAGAATGTGACTTCATCACCCAGCTGAGAGAGGATGAGAGTGCCTGTCTACAAGCAGCAGAGGAGATGCCCAACACCACCCTGGGCTGCCCTGCGACCTGGGATGGGCTGCTGTGCTGGCCAACGGCAGGCTCTGGCGAGTGGGTCACCCTCCCCTGCCCGGATTTCTTCTCTCACTTCAGCTCAGAGTCAGGGGCTGTGAAACGGGATTGTACTATCACTGGCTGGTCTGAGCCCTTTCCACCTTACCCTGTGGCCTGCCCTGTGCCTCTGGAGCTGCTGGCGTGAGGAGG
mRNA sequence
CAACAUGUAUGUGGCAACAUGGACCCAGAAUGUGACUUCAUCACCCAGCUGAGAGAGGAUGAGAGUGCCUGUCUACAAGCAGCAGAGGAGAUGCCCAACACCACCCUGGGCUGCCCUGCGACCUGGGAUGGGCUGCUGUGCUGGCCAACGGCAGGCUCUGGCGAGUGGGUCACCCUCCCCUGCCCGGAUUUCUUCUCUCACUUCAGCUCAGAGUCAGGGGCUGUGAAACGGGAUUGUACUAUCACUGGCUGGUCUGAGCCCUUUCCACCUUACCCUGUGGCCUGCCCUGUGCCUCUGGAGCUGCUGGCGUGAGGAGG
Protein sequence: (in this consider first three bases coded for amino acid.)
QHVCGNMDPECDFITQLREDESACLQAAEEMPNTTLGCPATWDGLLCWPTAGSGEWVTLPCPDFFSHFSSESGAVKRDCTITGWSEPFPPYPVACPVPLELLA&
GG
In this mutant, mRNA codes for 97 amino acids long polypeptide. In this protein, 32-56 amino acids motif represents the signature sequence G-protein coupled receptor family. These protein receptor family detect molecule present outside the cell and interact them and initiate internal signal transduction pathway/process.
Note : in this case first three bases include for coding of amino acid. There are other translation possibilities also. Suppose coding for first amino acid starts with 2nd base than overall protein sequence will be different. I am just giving one example (5 other combinations also possible)
Protein sequence: ( this example is just for knowledge)
NMVCGNMDPECDFITQLREDESACLQAAEEMPNTTLGCPATWDGLLCWPTAGSGEWVTLPCPDFFSHFSSESGAVKRDCTITGWSEPFPPYPVACPVPLELLA&
GG