Plasmid vectors are small, circular DNA molecules used in recombinant DNA proced
ID: 853532 • Letter: P
Question
Plasmid vectors are small, circular DNA molecules used in recombinant DNA procedures. A hypothetical plasmid, pSAP, is illustrated below. Label each feature of the plasmid with the phrase that best describes its function.
Part II
Suppose you have a DNA fragment you would like to insert into the pSAP plasmid. The fragment has PstI and EcoRI restriction endonuclease sites near the 5' end and HindIII and Smal restriction endonuclease sites near the 3' end. Choose the best restriction endonuclease(s) to digest the both the DNA fragment and pSAP.
Explanation / Answer
Ori = the site where DNA replication can begin
amp= a gene that allows cells to grow when exposed to certain conditions
Blue region = a region with multiple resrtiction sites....
EcoRI and HindIII