Please explain the function of the four oligonucleotides: 1. forward amplificati

Question

Please explain the function of the four oligonucleotides:

1.  forward amplification primer

2. reverse amplification primer

3. capture probe

4. signal probe

And, how can the assay be improved upon to be more specific? For example, if one virus is not able to be detected using this test, but a closely related virus is, and yields false positive results for the first virus, how can it be changed to better distinguish between the two viruses?

INNOVATIVE TECHNOLOGY Sensor ferrocene label 1 The target DNA is mixed with the signal probe solution. If the applicable target DNA is present, hybridization to the signal probes occurs immediately. Ferrocene Signal Bockground Cument 30 label -0.2 00 02 The solution is pumped through 3 Voltage is swept across the cartridge's microfluidic chamber each electrode and target and the target DNAVsignal probe DNA is analyzed by complex completes the reaction with electrochemical detection the pre-assembled capture probe.

Explanation / Answer

with the help of forward and reverse amplification primer, we will amplify the virus DNA sequence by PCR. by doing so we will get enough amount of target DNA molecule.

signal probe binds to the DNA sequence and stabilises it. also, it contains fluorescent tag which will not give any fluorescence until detached from the DNA.

now capture probe has more affinity towards the DNA sequence that's why it competes with the signal probe and removes it from DNA. by application of voltage, we activated the fluorescent group present on the signal probe which can be detected by the detector.

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