Identify the most likely technique error that lead to each of the following outc

Question

Identify the most likely technique error that lead to each of the following outcomes. Spots were smeared out vertically, appearing as ill-defined blobs rather than nice round spots. No spots appeared on the plate after visualization. Solvent did not run evenly past the marked baseline. Random spots were observed all over the plate. Neighboring spots overlap horizontally, appearing as ill-defined blobs rather than nice round spots. Solvent level was above baseline, dissolving the sample. Too much sample was applied (too concentrated). Stationary phase scraped off by pencil. Spots were too large. Plate was dropped on bench or touched, contaminating the stationary phase

Explanation / Answer

1. Spots were smeared out verically, appearing as ill-defined blobs rather than nice spots- too much sample was applied.

2. No spots on plate after visualization- solvent level was above baseline, dissolving the sample.

3. Solvent did not run evenly past the marked baseline- stationary phase scraped off by pencil.

4. Random spots were observed all over the plate- plate was dropped on bench or touchhed contaminating the stationary phase.

5. Neighbouring spot overlap horizontally appearing as ill defined blobs rather than nice spots- spots were too large.

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