Identify the role of the following DNA replication by matching them with their d
ID: 8728 • Letter: I
Question
Identify the role of the following DNA replication by matching them with their descriptions.Components i.gyrase
ii.helicase
iii.SSBs
iv.DNA polymerase III
v.leading strand
vi.DNA polymerase I
vii.DNA ligase
viii.Okazaki fragments
Description of roles
a. catalyzes phosphodiester bonds between adjacent fragments
b. adds DNA to the leading and lagging starnds while checking for and repairing errors
c. joins independent fragments of DNA on the lagging strand by phosphodiester bonds
d. breaks down hydrogen bonds between base pairs
e. is established by beginning replication at a 3' end, where an RNA primer has been attatched.
f. prevents unwound parent template strands from winding back together.
g. are short DNA sequences that form the lagging strand.
h. unwinds the double helix.
i. lays down RNA primers needed by DNA polmerase III to add DNA nucleotides to a complementary strand.
j. replaces RNA primers on the lagging strand with DNA bases.
Can you please match the above?
Explanation / Answer
i)
ii) h
iii) f
iv) b
v) e
vi) j
vii) c
viii) g
Now the reason I didn't include the answer to number one is that it's slightly confusing. Gyrase is a topoisomerase, which on a basic level means it relieves the supercoiling effect of DNA replication upstream of DNA Polymerase III and helicase. It does this by breaking the phosphodiester bonds in the backbone of one of the DNA strands to relieve the supercoiling and then reconnect the relaxed strands.
The only 3 remaining options are a, d, and i, and I wouldn't say any of those fit the description of what gyrase does. Now I'm not sure what you're instructor is teaching, but it's possible that they were looking for you to say that helicase breaks down hydrogen bonds between base pairs (which is most CERTAINLY does), and say that gyrase is what unwinds the double helix, which while I would say is the closest answer to gyrase, still doesn't make much sense. Plus if you asked any biologist what enzyme unwinds the DNA helix during replication, 99% of them would immediately answer "helicase."
Sorry I couldn't be clearer on number 1, but a quick internet search of DNA gyrase or even topoisomerase will give you a brief description that might help you in making a decision.
Good Luck!!