The following DNA sequence represents a eukaryotic gene. Indicate which is the t
ID: 87981 • Letter: T
Question
The following DNA sequence represents a eukaryotic gene. Indicate which is the template and which is the coding strand, determine where transcription begins (assume it ends at the end of the sequence presented), and write out the nucleotide sequence of the initial transcript. You need to process the initial transcript to make an mRNA (there is an intron in the gene; using the information about splicing on pp. 306-307 of your textbook, locate and remove the intron) and write out the sequence of the completely processed mRNA. Finally, translate the mRNA and write out the amino acid sequence of the encoded polypeptide. 5’ACCGGCCTCTCTCTCACACACAGTACGGCGTTTATTGGGACTTCAAATCATATGGCCATTACAAGCCCTGCAGAAAGGGAAGGCGAAGCTCAGGATAAGTA... 3’-TGGCCGGAGAGAGAGTGTGTGTCATGCCGCAAATAACCCTGAAGTTTAGTATACCGGTAATGTTCGGGACGTCTTTCCCTTCCGCTTCGAGTCCTATTCAT... ...CAGGTGACACTCTACTCACCGGATAAGTATTGTCATCTCCCAAGGGGGTGAATATCCGCGCATCCGCTTTATATCGCCTGATTAACGCAGTCCTACACAAT-3’ ...GTCCACTGTGAGATGAGTGGCCTATTCATAACAGTAGAGGGTTCCCCCACTTATAGGCGCGTAGGCGAAATATAGCGGACTAATTGCGTCAGGATGTGTTA-5’
Explanation / Answer
Coding strand:
The base sequence of the opposite strand directly corresponds to the codons in the mRNA and is called as coding strand.Here the coding strand is:
Coding strand
5’ACCGGCCTCTCTCTCACACACAGTACGGCGTTTATTGGGACTTCAAATCATATGGCCATTACAAGCCCTGCAGAAAGGGAAGGCGAAGCTCAGGATAAGTA... 3’-
Template strand:
The strand that is copied into mRNA and subsequently translated has the complementary sequence to the mRNA, is called as template strand. Here the template strand is:
Template strand
TGGCCGGAGAGAGAGTGTGTGTCATGCCGCAAATAACCCTGAAGTTTAGTATACCGGTAATGTTCGGGACGTCTTTCCCTTCCGCTTCGAGTCCTATTCAT...