Please answer the following three questions involving this enzyme purification s
ID: 887121 • Letter: P
Question
Please answer the following three questions involving this enzyme purification scheme:
Fraction
Vol (mL)
Protein (mg)
Activity
Specific Activity
Crude lysate
200
2000
2646
1.32
A) differential centrifugation
200
1000
1323
1.32
B) Heat treatment
200
500
1200
2.4
C) Ion exchange
10
60
1100
18.3
D) Size exclusion
60
30
1000
33.3
E) Heparin Affinity
10
20
800
40
What step resulted in the largest loss of the enzyme of interest?
Fraction
Vol (mL)
Protein (mg)
Activity
Specific Activity
Crude lysate
200
2000
2646
1.32
A) differential centrifugation
200
1000
1323
1.32
B) Heat treatment
200
500
1200
2.4
C) Ion exchange
10
60
1100
18.3
D) Size exclusion
60
30
1000
33.3
E) Heparin Affinity
10
20
800
40
Explanation / Answer
I think you need to see the difference between specific activity of enzyme. If difference is more,it has resulted in largest lost of enzyme.
Ion exchange has largest difference from previous one which is 18.3-2.5 = 15.9
So answer : C) Ion exchange