In the CRISPR/Cas9 nuclease system, what is the role of the sgRNA? a) The sgRNA
ID: 90925 • Letter: I
Question
In the CRISPR/Cas9 nuclease system, what is the role of the sgRNA?
a) The sgRNA is required to pair the CRISPR/Cas9 with its intended target site in the RNA and to activate the CRISPR nuclease domains for cleavage. b) The sgRNA is required to pair the CRISPR/Cas9 with its intended target site in the DNA and to activate the CRISPR nuclease domains for cleavage. c) The sgRNA is required to pair the CRISPR/Cas9 with its intended target site in the DNA and then cleaves one or both of the CRISPR nuclease domains. d) The sgRNA is required cleave both of the CRISPR nuclease domains so it will then bind the intended target site in the DNA.Explanation / Answer
Correct Answer :- Option b
Explanation:- A single guide RNA (sgRNA) is generated to direct the Cas9 nuclease to a specific genomic location. A single guide RNA (sgRNA), consists of a crRNA sequence that is specific to the DNA target, and a trRNA sequence that interacts with the Cas9 protein. sgRNA binds to a recombinant form of Cas9 protein that has DNA endonuclease activity. The resulting complex causes target-specific double-stranded DNA cleavage.
Option a metions intended target site for CSIPR/Cas9/sgRNA system as RNA, which is not correct. Intended target site is DNA. Therefore, the option a is inccorrect.
Option c and option d mention cleavage of CSIPR nuclease domains. The sgRNA helps CSIPR endonuclease to bind and cleave DNA. sgRNA does not cleave the nuclease itself. Thus option c and option d are incrorrect.