Map pling A) Choose the factors that lead to asymmetry in chromatographic peaks

Question

Map pling A) Choose the factors that lead to asymmetry in chromatographic peaks High column temperature Strong interaction between the solute and some sites of the stationary phase High flow rate Low column temperature Large number of possible flow paths through the column Overloading the column with solute B) A separation was performed through an 23 cm-long column that generated a chromatogram with an asymmetric peak in 16 min (t). The asymmetric peak had a width at 1/10 of the height (w01 of 35 s with an A component of 8 s. Determine the plate height for this peak in millimeters. Number Plate height

Explanation / Answer

we know that

W0.1 = peak width at 1/10 height = A + B

given

W0.1= 35

A = 8

so

B = 35 - 8 = 27

now

we know that

N = 41.7 (tR/W0.1)^2 / (A/B + 1.25)

given

tr = 16 min = 960 s

so

N = 41.7 x ( 960/ 35)^2 / [ ( 8 / 27) + 1.25 ]

N = 20288.49

now

N = L / H

given

length (L) = 23 cm

so

20288.49 = 23 / H

H = 1.133 x 10-3 cm

H = 1.133 mm

so

the plate height is 1.133 mm

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