Map pling A) Choose the factors that lead to asymmetry in chromatographic peaks

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Map pling A) Choose the factors that lead to asymmetry in chromatographic peaks Strong interaction between the solute and some sites of the stationary phase High column temperature ow column temperature Overloading the column with solute High flow rate Large number of possible flow paths through the column B) A separation was performed through an 23 ct ong column that generated a chromatogram with an asymmetric peak in 16 min (t). The asymmetric peak had a width at 1/10 of the height (w01 of 35 s with an A component of 8 s. Determine the plate height for this peak in millimeters. Number Plate height Tools x 1

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Map pling A) Choose the factors that lead to asymmetry in chromatographic peaks

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