Carbonic anhydrase (molar mass = 30,232 g/mol) of red blood cells has one of the

Question

Carbonic anhydrase (molar mass = 30,232 g/mol) of red blood cells has one of the highest turnover numbers. It catalyzes the reversible hydration of CO2: H2O + CO2 H2CO3  

If 15.5 µg of pure carbonic anhydrase catalyzes the hydration of 0.478 g of CO2 in 1.00 min at 25°C at Vmax, what is the turnover number (kcat) of carbonic anhydrase? The units should be in min-1.

b. If the Km of carbonic anhydrase for the above reaction is 4.81 mM, what is the catalytic efficiency of this enzyme under these conditions? The units are min-1M-1.

Explanation / Answer

Vmax= 0.478/1= 0.478g/min

Turn over number= Vmax/ Et where Et =Concentration of Enzyme= 0.478/(15.5*10-6)=30838.71/min

b. Catalytic efficiecy= 1/Km* turn over number= 100*1/30838.71*4.81*10-6)=14.8/minM-1

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