An oligomeric protein has a molecular weight of 333000. A 770 mg sample was trea
ID: 950601 • Letter: A
Question
An oligomeric protein has a molecular weight of 333000. A 770 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. The product of the complete hydrolysis contained 2.3 mg of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the alpha-amino groups of other amino adds could be found. How many polypeptide chains are in this protein? (Enter your answer as a whole number).Explanation / Answer
335 polypeptide chains are present in this protein