Please answer questions 3 and 4. All the information you might need is contained
ID: 953359 • Letter: P
Question
Please answer questions 3 and 4. All the information you might need is contained in the theory and procedure.
PRE-LAB ASSIGNMENT 1. Predict the product of the reduction reaction with benzofuran-2-yl methyl ketone. carrot fragments H2O, room temp O O Will the expected product be optically active? Why or why not? 3. that two reagents are typically employed to carry out the synthetic transformation performed in this lab? Look up and reference the MSDSs for both and comment on their human toxicity and stability and reactivity 4. What is column chromatography? How could it be employed in this experiment? 5. In the micro-extraction with ethyl acetate, which layer will be on the top (with proof and reference)? Which layer will contain the crude product? 6. Look up and reference the MSDS for benzofuran-2-yl methyl ketone, ethyl acetate and hexane and comment on the human toxicity Draw a "to-scale" replicate of the two TLC plates that you will be preparing in this experiment. Which compound, reactant or product, will be found higher up on the silica plate during TLC analysis? Explain your answer Why are the vegetables being cut with a knife only and not blended in this experimen B.Explanation / Answer
3)
Two important reagents that can be usedd for artificial synthesis of alcohol from Benzofuran-2-yl methyl ketone
A) Lithium aluminium hydride(LiAlH4) and B) Sodium borohydride(NaBH4)
Their humentoxicity, stability, and reactivity are as follows:
A) LiAlH4:
a) Stability and reactivity:
i) Product is stable
ii) Instable with water, heat, acid explosively rective
iii) On burning produces obnoxius and toxic gases.Water contact generates flammable gas.
b) Toxicity to human:
i) Hazardous if coms in contact with skin, eyes. Irritant to skin, eye, dameges skin tissues,Inflammation and blistering of skin. May cause corneal damage on eye contact.Inhalation of dust produced on accidental burning cause gastro intestinal irritation.Carcinogenic, mutagenic, teratogenic effects and developmental toxicity not observed.
=========
B) NaBH4:
a) Reactivity and stability:
i) Stable
ii) Instability temperature and condition of instability not available.
iii) Extremely reactive with oxidants, acids, alkalies, moisture.
b) Human toxicity: almost same as that of LiAlH4.
===============
4)
i) Column chromatography: In column chromatography stationary phase is placed in a narrow column through which the mobile phase moves under the enfluence of gravity(earth gravity) or pressure. The stationary phase is either solid orthin, liquid film coating on a solid particulate material or on walls of column.
Column chromatography has wide application in small as well as large scle separation. Many antibiotics are purified by using column chromatography.
Stationary phase : Alumina and silica are most popolar stationary phases.
Solvent : initially non-polar or slightly polar solvent and then gradually polarity of solvent is increased.
Application of Column chromatography in given experiment:
The above organic reaction involves conversion of aromatic ketone into correspponding aromatic alcohol through reduction of carbonyl group. The reaction uses water, reductases (Biological reducing agents). The reaction mixture involves starting ketone, product etc and other molecules in the carroot fragments.
The separation of product from all these molecules can be efficiently done with column chromatography.
Hence the use of CC in the experiment.