This diagram is used for questions A-F. I got questions A,B,D,E,and F but stumpe
ID: 98448 • Letter: T
Question
This diagram is used for questions A-F. I got questions A,B,D,E,and F but stumped on C.....Question C: What end (5' or 3') of the molecule is indicated by arrow NUMBER 8? Which strand of DNA is indicated by NUMBER 7? What kind of nucleic acid is indicated by arrow NUMNBER 4? What do you call the short DBA fragments indicated by arrow NUMBER 5? What enzyme functions to join the short fragments indicated by arrow NUMBER 6? which enzyme is indicated by arrow NUMBER 3? USE THE DIAGRAM BELOW TO ANSWER THE FOLLOWING QUESTIONS (A-F) 2. (which enzyme deals with supercoils?) . (5' or 3' end?) 7. (leading or 6. (which enzyme joins the nick?) 5. 4. 3. (which enzyme?) 8. (which end 5' or 3)
Explanation / Answer
Answer C (i): Arrow NUMBER 8 is a 3’ end of the lagging stand (3’ to 5’), the direction of this strand is opposite of that of replication fork.
Answer C (ii): Arrow NUMBER 7 indicates leading strand (5’ to 3’). Leading strand is synthesized along the direction of replication fork.
Answer C (iii): Arrow NUMBER 4 represents DNA polymerase III. It catalyzes nucleotide addition to the substrates of DNA in the 5' to 3' end direction.
Answer C (iv): Arrow NUMBER 5 is called primer. It is a short strand that serves as start point for synthesis of DNA. It works in tandem with DNA polymerase, since DNA polymerase can only add the nucleotides to the already existing DNA strand.
Answer C (v): DNA ligase is the enzyme that functions to join the short fragments also called as Okazaki fragments at lagging point during DNA replication, indicated by arrow NUMBER 6.
Answer C (vi): Arrow NUMBER 3 indicates enzyme helicase. Helicase enzyme splits the two strands of the DNA at replication fork during DNA replication.