Identify the group in pyridoxal phosphate (PLP) that covalently binds enzyme and
ID: 989768 • Letter: I
Question
Identify the group in pyridoxal phosphate (PLP) that covalently binds enzyme and substrate. Aminotransferases (transaminases) must deaminate one amino acid and add an amino group to another amino acid during transamination. How do the two processes differ? The coenzyme PLP must be activated by an external amino group before it can catalyze the removal of the amino group from the first amino acid. The second part of the reaction (adding an amino group) is the reverse of the first (deamination). The first reaction involves the formation of a Schiff base, whereas the second does not. The second part of the reaction takes place at a separate amino acid residue in the active site.Explanation / Answer
(a) The group which binds to the enzyme and substrate in pyridoxal phosphate (PLP) is,
B
(a) The deamination process occruing in two steps differ as,
The coenzyme PLP must be activated by an external amino group before it can catalyze the removal of the amino acid group from the first amino acid.