Match each of the following affinity chromatography terms with its definition or
ID: 990894 • Letter: M
Question
Match each of the following affinity chromatography terms with its definition or description. Biospecific elution Affinity ligand Application buffer General ligand Nonspecific elution High-specificity ligand Immobilization method Elution buffer A solvent that is used to displace the analyte from the immobilized molecule. It binds to a class or family of related molecules. It is one of a pair of interacting molecules that is immobilized onto the column support. In this technique, the association equilibrium constant between the analyte and immobilized ligand is decreased through a change in pH, ionic strength, or polarity. A solvent that mimics the pH, ionic strength, and polarity of the immobilized molecule's natural environment. It binds to one or a few closely related molecules. This process typically involves covalently coupling of the molecule to the support through amine, carboxyl, or sulfhydryl groups on the molecule. In this technique, a competing agent is added to the mobile phase to displace the analyte from the immobilized molecule.Explanation / Answer
1 - elution buffer
2 - general ligand
3 - high specific ligand
4 - nonspecific elution
5 - application buffer
6 - affinity ligand
7 - biospecific elution
8 - immobilized method