Match each of the following affinity chromatography terms with its definition or
ID: 991633 • Letter: M
Question
Match each of the following affinity chromatography terms with its definition or description.
Elution buffer, General ligand, Affinity ligand, Immobilization method, Application buffer, High-specificty ligand, Biospecific elution, Nonspecific elution.
___ A solvent that is used to displace the analyte from the immobilized molecule
___ It binds to a class or family of related molecules
___ It is one of a pari of interacting molecules that is immobilized onto the column support
___ This technique, the association equilibrium constant between the analyte and the immobilized ligand is decreased through a change in pH, ionic strength, or polarity.
___ A solvent that mimics the pH, ionic strength, and polarity of the immobilized molecule's natural environment
___ It binds to one or a few closely related molecules
___ This process typically involves covalently coupling of the molecule to the support through amine, carboxyl, or sulfhydryl groups on the molecule
___ In this technique, a completing agent is added to the mobile phase to displace the analyte from the immobilized molecule
Explanation / Answer
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Elution buffer solvent that is used to displace the analyte from the immobilized molecule
General ligand It binds to a class or family of related molecules
High-specificty ligand It is one of a pair of interacting molecules that is immobilized onto the column support
Immobilization method This technique, the association equilibrium constant between the analyte and the immobilized ligand is decreased through a change in pH, ionic strength, or polarity.
Application buffer that mimics the pH, ionic strength, and polarity of the immobilized molecule's natural environment
Affinity ligand It binds to one or a few closely related molecules
Biospecific elution This process typically involves covalently coupling of the molecule to the support through amine, carbox
Nonspecific elution in this technique, a completing agent is added to the mobile phase to displace the analyte from the immobilized molecu