Match the terms with the correct definition. 1) An enzyme that recognizes the DN
ID: 99185 • Letter: M
Question
Match the terms with the correct definition.
1) An enzyme that recognizes the DNA sequence of GAATTC and cuts the DNA between the G and At the A.
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
2) Enzymes that cleave the DNA at specific sites within the DNA
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
3) A chemical or physical agent that causes the DNA to mutate
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
4) An enzyme the "fixes" breaks in the DNA sugar-phosphate bacbone
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
5) An enzyme that recognizes the DNA sequence of AAGCTT and cuts the DNA between the two A's.
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
6) A protein that is produced by one bacteria that is toxic to another
a- EcoRI
b- Restriction Endonucleases
c- HindIII
d- DNA ligase
e- Mutagen
f- Bacteriosin
7) Dr. Masano was performing research to deal with the problem of the cassava mosaic disease that was reducing yields of cassava in Zimbabwe. What was the direction that he was taking to reduce the effects of this disease?
a) He was trying to get the plants to naturally produce an insecticide that would kill the insect that was carrying the pathogen.
b) He was trying to train people to get by on eating less cassava
c) He was trying to create a "vaccine" to make the cassava plants immune to the pathogen that caused the mosaic disease.
d) He was engineering bigger plants that could easily beat up the pathogen
e) He was mutating the existing DNA of the plants so that the pathogen could not infect the plants
8) In zimbabwe a plant pathogen was causing problems with the cassava crop, which was a source of food for the people living int hat country. What was that plant pathogen that was causing the problem?
a) The zimbabwean mosiac bacteria
b) The zimbabwean mosaic virus
c) The cassava-killing bacteria
d) The cassava killing virus
e) The acacias mosiac virus
f) the cassava mosaic fungi
g) the cassava mosaic bacteria
9) What bacterium was frederick griffith using when he reported transformation in bacteria?
a) Staphylococcus
b) Corynebacterium
c) campylobacter
d) streptococcus
e) Haemophilus
f) transformia
g) Neisseria
10) What was responsible for the spread of the pathogen that was causing problems with the cassava crop in Zimbabwe?
a) Mosquitoes
b) human contact
c) the wind
d) wild animals and small children
e) white flies
Explanation / Answer
Answer
In genetic engineering, restriction endonucleases serve as a tool for cutting DNA at specific sites. Eco R1is a widely used restriction endonuclease enzyme that cuts DNA molecules at predetermined sites (GAATTC) between bases G and A.
In Prokaryotes, there is a special class of enzymes which cut DNA at specific site, these are called Restriction Endonucleases.
Correct Answer is (b)
Mutation (a base-pair sequence change within the DNA of a gene) occurs in spontaneous rate in nature. Mutation can also be induced artificially with the help of some physical or chemical agents. These agents are called Mutagens.
Correct answer is (e)
4) An enzyme the "fixes" breaks in the DNA sugar-phosphate bacbone
DNA ligase is an enzyme that closes nicks in one strand of double-stranded DNA by creating an ester bond between adjacent 3' OH and 5' PO4 ends on the same strand. DNA ligase is found in prokaryotic cells, eukaryotic cells, viruses and bacteriophages.
Correct answer is (d)
HindIII is restriction endonuclease that is specific for site 5-A/AGCTT-3 to generate DNA fragments with cohesive 5-ends.
Correct answer is (c)
Many bacteria and archaea produce Bacteriosin, a ribosomally synthesized antimicrobial peptides that inhibit the invasion of competing strains or pathogens. Thease also modulate the composition of the microbiota and influence the host immune system.
Correct answer is (f)
Dr. Masano was trying inhibition via RNA interference should constitute a useful method for generating cassava mosaic virus-resistant plants.
Correct answer is (c)
8) In zimbabwe a plant pathogen was causing problems with the cassava crop, which was a source of food for the people living int hat country. What was that plant pathogen that was causing the problem?
Cassava, a vegetatively propagated is the most important crop of a South Africa it is particularly vulnerable to viruses cassava mosaic disease (CMD) and thus lead to great economic loss each year.
Correct answer is (d)
9) What bacterium was frederick griffith using when he reported transformation in bacteria?
Frederick Griffith, a renowned bacteriologist used Streptococcus pneumoniae in his transformation experiment.
Correct answer is (d)
10) What was responsible for the spread of the pathogen that was causing problems with the cassava crop in Zimbabwe?
Whitefly are the main cause to become a crop pest causing damage to cassava leaves as well as being a disease vector
Correct answer is (e)