Count the number of base pairs for each fragment. Remember that a base pair incl
ID: 101786 • Letter: C
Question
Count the number of base pairs for each fragment. Remember that a base pair includes two bound nucleotides. If a base is not paired because of a sticky end, do not count it. This count determines the approximate size of the fragment.
Wild-Type:
5’ CTG ACT CCT GAG 3’
3’ GAC TGA GGA CTC 5’
Homozygous Mutant:
5’ CTG ACT CCT GTG 3’
3’ GAC TGA GGA CAC 5’
a. For the Ddel-digested, wild-type beta-globin sequence, how many nucleotides are in each fragment?
b. For the Ddel-digested, mutant beta-globin sequence, how many nucleotides are in each fragment?
Explanation / Answer
12 base pairs in each (wild and mutant) (no sticky ends)
a, b each has 12 nucleotides