Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and
ID: 1026666 • Letter: D
Question
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Describe how you would prepare 2mL of solution containing NaCl, MES buffer, and water. NaCl stock solution is 1M and the working concentration required is 5mM. MES buffer stock solution is 100M and the working concentration required is 20mM, To make 2mL of solution calculate the amount of water you would add.
Explanation / Answer
V1N1=V2N2
For NaCl,
0.005*2=1*x
therefore x=0.010 ml of Nacl
For buffer,
0.02*2=100*y
y=0.0004 ml of Buffer
remaining is 2-(x+y)
Water to be added is 2-0.0104 =1.9896 ml