Please answer nucleophilic substitution post lab questions Explain how the amoun
ID: 1058149 • Letter: P
Question
Please answer nucleophilic substitution post lab questions Explain how the amount of sample injected onto a column affects the separation of a two-component mixture. What effect would each of the following have on the retention times? a. increase the column temperature b. increase the flow rate of the carrier gas c. use a more polar column A student neglected to add sulfuric acid to the reaction mixture. What is the expected outcome? Explain. A possible side product from the experiment is dibutyl ether. Where in the procedure is this compound removed? Explain where the alkenyl side products arc removed in the experimental procedure.Explanation / Answer
1. If the sample injected into the column is too concentrated we would not get good resolution or separation of components on the column. The sample has to be dilute enough for good separation.
2. Effect of following on retention times,
a. Increase in column temperature would give lower retention times as compounds would elute fast without much retention on column.
b. Increase in flow rate of gas, would give shorter retention time as compounds would elute faster without much retention on the column.
c. If a more polar column is used, polar components would be retained much longer and non-polar's would elute faster.
3. Without sulfuric acid, no reaction and no product formation would occur. We would get starting material back.
4. Dibutyl ether has a lower boiling point and is removed by distillation.
5. Alkenyl side products are removed either by distillation or by column chromatography of the reaction mixture.