Please answer.. thanks Part 1. cDNA synthesis and amplification by Reverse Trans

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Please answer.. thanks

Part 1. cDNA synthesis and amplification by Reverse Transcription-PCR Below is the genomic DNA sequence of a simple eukaryotic gene that encodes a 7-amino acid polypeptide Transcription starts at the first T. The translation start and stop codon are underlined. 5,_ T A T G C A G T A T C A G C A T G C G C A T A A T CC-3, 3'- A T ACGTcATAAGTCGTAC G CGT A TEA GG5 1. Write out the sequence of the primers needed to synthesize a cDNA that contains the open reading frame including the stop codon. The primers should be 10 nucleotides long. Label the 5' and 3' end of your oligonucleotide primers. I 1 point Sequence of Forward primer: 1 point Sequence of Reverse primer:

Explanation / Answer

Complementary stand: 5' T A T G C A G T A T T C A G C A T G C G C A T A A T C C 3'

suppose we are taking about 5 bp primer.

so forward primer will be: 5' TATGC 3'

& reverse primer will be: 3' CCTAA 5'

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