Please answer the following multiple-part question regarding vectored cloning: a
ID: 142532 • Letter: P
Question
Please answer the following multiple-part question regarding vectored cloning:
a) You probably should use _________ vector to clone PCR-amplified DNA and ________ vector for making a genomic DNA library.
A. PCR TOPO TA/ pBAC
B. pUC18/pCR-TOPO TA
C. C. Lambda GT11/ YAC
D. pBAC/lambda GT11
b) You want to use _______ vector to clone short DNA fragments for DNA sequencing and ______ vector for cDNA library construction.
A. pUC18/ Lambda GT11
B. Lambda GT11/PCR TOPO TA
C. PCR TOPOTA/shuttle vector
D. shuttle vector/ YAC
c) A promoter, a terminator, a RBS, a start codon and one or more stop codons are built around the MCS of the ___________ vectors?
A. pBAC and pYAC
B. pUC18 and pCR TOPO
C. vectors used in library construction
D. vectors used in gene expression in bacteria
d) Why are genomic DNA libraries made using BAC vectors and not YAC vectors even though the YAC vector may clone extremely large DNA fragments?
A. Inserting YAC vectors inside the cells is extremely difficult
B. DNA cloned in YAC vectors may get lost because of recombination
C. DNA cloned in YAC vectors gets lost because YAC vectors are unstable
e) The cloning vectors used for human gene therapy are mainly based on _________.
A. bacterial viruses (i.e. phages)
B. animal viruses
C. plasmids
D. BACs
E. YACs
Explanation / Answer
A. PCR TOPO TA/ pBAC
B. pUC18/ Lambda GT11
C. vectors used in gene expression in bacteria
D. DNA cloned in YAC vectors may get lost because of recombination
E. animal viruses