See the experiment in the image, testing for Carbon dioxide production. 1. For e
ID: 148248 • Letter: S
Question
See the experiment in the image, testing for Carbon dioxide production.1. For each test tube, what results do you expect and why?
2. How many variables are in the experiment?
3. Ideally, how many variables are different between the control and an experimental group in an experiment? Protocol: A. Obtain seven 13x100 test tubes and label 1-7. B. Add the solutions listed in the bolded columns of the table below. NOTE: NaF is a poison, wear goggles and gloves when handling NaF. et-up and data collection table CO Fill to the top with Yeast Yeast 0.1 M Tube pyruvate MgSO 3 M 0.1 M NaF | 5% glucose | produced Water in mm 7.5 mL 2.5 mL 5.0 mL 2.5 mL Yeast Yeast Yeast Yeast 5.0 mL 0.5 mL2.5 mL 4.5 mL 5.0 mL2.5 mL 2.5 mL 2.5 mL 6 2.5 mL 2.5 mL2.5 mL Water C. After adding the specified volumes of pyruvate, MgsO, NaF, gucose, and water, fill the tubes completely to the top with the solution indicated in the table. For tubes 1-6, fill to the top with yeast suspension, and for tube 7, fill to the top with water. D. Obtain seven 16x100 test tubes. For each of the seven tubes you filled, slide one of the larger test tubes completely over the top, covering the entire tube. Hold the filled inner tube firmly against the inside botton of the larger tube, and invert the assembly (lip the tubes upside down). If done properly, the contents of the inner test tube will stay inside it even after inversion.
Explanation / Answer
Answer:
1: Test tube order for CO2 production
High to Low CO2 production (Tube No): 3 >6>5>4>2>1>7
Tube 3: Highest production (because organic substrate glucose and MgSO4 are present with yeast )
Tube 6: Two organic substrate pyruvate and glucose and NaF with yeast causing more respiratory organic molecule but MgSO4 absent which is essential for the metabolic activity of Yeast.
Tube 5: NaF and glucose present but water is not added and NaF has more concentration. so more nutrients concentration for yeast
Tube 4: NaF and glucose present but water added and low concentration of NaF than tube 5
Tube 2: only organic substrate glucose and water is present for yeast so expecting less production
Tube1: Water with yeast cell. The cell will be less active, since no nutrients present. The lowest amount of CO2 will be produced.
Tube 7: No production because there are no yeast cells (no inoculum)
2: Number of variables in the experiment is 6 ( 5 input nutrients, 1 outcome CO2)
3. 3 different types of the variable (dependent, independent and control)