I just need help with queation #19. You have to use the word bank to fill in the

Question

I just need help with queation #19. You have to use the word bank to fill in the blanks.

For questions 16-19, please use the word bank in the box below to fill in the spaces. mRNA, RISC, cDNA, gDNA, PCR, RNAase-H, drosha, viral, non-viral, hydrogen, 5SoC, 720C, 9SoC, two- fold, PCR, reverse transcription, primer, reverse transcriptase, Poly-A tail, luciferase, dNTP, positively, negatively, RNA Polymerase, ATP, dicer, guide RNA, endocytosis, ATP sulfurylase, apyrase, APS, luciferin, pyrophosphate, luciferase

Explanation / Answer

1) detection of released pyrophosphate during DNA synthesis

2)Cycles of four dNTP separately added

3)inorganic pyrophosphate released

4)Released pyrophosphate subsequently converted to ATP by ATP sulfurylase in the presence of APS, which drives the conversion of luciferin to oxyluciferin in the presence of enzyme luciferase.

5) Apyrase is nucleotide degrading enzyme

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