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ID: 151188 • Letter: I
Question
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APPENDIX 5 Calibration and Collecting Absorbance Data for the Vernier Spectro Vis Plus Spectrophotometer This page is for coll Lab ecting a spectrum analysis for the Data Anabysis Lah and the Phatosnthesis Do not touch the smooth surfaces of the cuvette. Handle only by the rough surfaces. Use a Kim Wipe to gently wipe the smooth surfaces before proceeding. 1. Connect the Vernier Spectro Vis Plus spectrophotometer to a USB port on your group's laptop 2. Click on the Logger Pro icon on the computer desktop. 3. Insert a cuvette containing the appropriate blank (solvent) into the Spectro Vis. Smooth surface of the cuvette toward white light symbol. 4. Click on the Experiment button in the menu bar 5. Drop down to Calibrate and select Spectrometer 6. If this is the first time you are using the Spectro Vis, allow it to complete its warm up. 7. When it says warm up complete, click Finish Calibration. Then, when the OK button is available, click it. 8. Insert the sample for which you want to collect absorbance readings at different wavelengths of visible light. 9. Click the Collect (green play button in menu bar). 10. A graph of showing the absorbance of the extract at different wavelengths will be displayed. Click Stop to end the data collection. II. Your spectrum analysis curve should appear overlaying a visible light spectrum. The absorbance at individual wavelengths will appear on the left side of the screen. 12. 13. You can print these dataExplanation / Answer
Vernier SpectroVis is a portable, affordable visible light spectrophotometer which has fluorescence capabilities. Measurable wavelength range is 380-950 nm, and coloured results are analyzed. It follows Beer's law.
1. The spectrophotometer was connected to a USB port on a laptop.
2. Logger Pro icon on the laptop was clicked.
3. Cuvette containing appropriate blank (solvent) was inserted into it, with smooth surface of the cuvette towards white light symbol.
4. Experiment button was clicked in the menu bar.
5. Drop down to calibrate and spectrometer was selected.
6. In case of performing for the first time, it was allowed to warm up.
7. After warming, Finish calibration option was to be clicked.
8. The sample was inserted, absorbance was taken in different wavelengths of visible light.
9. Click the collect option.
10. A graph showing the absorbance data was shown at different wavelengths. stop option was clicked to finish data collection.
11. The spectrum analysis curve should appear overlaying a visible light spectrum. The absorbances at different wavelengths were shown on left.
12. If the spectrum is too small or on top off the screen, then adjust Axes button is to be clicked.
13. Data can be printed out.