Answer the following question using the databases( https://www.ncbi.nlm.nih.gov/
ID: 162821 • Letter: A
Question
Answer the following question using the databases(https://www.ncbi.nlm.nih.gov/guide/all/#databases) and search tools at NCBI(https://www.ncbi.nlm.nih.gov/ncbisearch/): What three genes in C. elegans encode proteins very similar in sequence to human pancreatic elastase 1? To make the task of answering the question easier, note the following.
a) Consider the protein's full name to be "Chymotrypsin-like elastase family member 1".
b) Do not consider any isoforms of the human protein.
c) Use a tblastn search.
d) Search against the "nr" database for C. elegans.
e) Use the default BLAST search parameters as determined by NCBI.
f) Read relevant sections of the "Entrez Help Document"(https://www.ncbi.nlm.nih.gov/books/NBK3837/) and the BLAST Help documentation(https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs)
g) Do not consider any hits on cosmids of C. elegans.
g) Consider only hits that have query coverage over 80%.
In your answer, give the names of the C. elegans genes. Also, describe how you came up with your answers. Illustrate that description with the results of the Entrez searches, BLAST searches, and database accesses which you performed. Use "screen dumps" from your web browser to show those results. Have the screen dumps in either PostScript (.ps file) or PDF (.pdf file) form. Marks will be given for the clarity of your description.
Explanation / Answer
The Caenorhabditis elegans genome sequence is now telomere 100,291,840 no. The per base error rate has been estimated at <(1/10^5).the genome seems to be in good shape.
Names of C elegans genes
M79.bb;W09B12.1(C elegans ACE -1 protein); Y44E3A.2;EGL-18;LET-2;SUP-10....... are some of the C elegans genes